Fig. 3

Presence of GFP-positive cells in the kidney of five-sixths nephrectomized mice. a As detected by FACS, the presence of GFP-positive cells peaked at 2 days and declined at 7 days after injection. After 28 days, few GFP-positive cells were detected in the kidney. Myh9-targeted mutant mice were used as a positive control and five-sixths nephrectomized mice treated with saline were used as a negative control. Data presented as mean ± SD. b–e Representative photomicrographs of GFP-stained kidney section 2 days and 14 weeks of CKD in mice treated with MKPCs. Kidney from Myh9-targeted mutant mouse was used as a positive control b. GFP-positive cells were found in some areas of the interstitium at the second day c, formed a tubule-like structure in the medulla d (inlet with higher magnification), or remained in the interstitium at 14 weeks. Scale bar: 50 μm. d days, GFP green fluorescent protein, h hours, M9 Myh9-targeted mutant mice, Saline five-sixths nephrectomized mice treated with saline