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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Regionally-derived cell populations and skeletal stem cells from human foetal femora exhibit specific osteochondral and multi-lineage differentiation capacity in vitro and ex vivo

Fig. 2

Multi-lineage differentiation potential of epiphyseal and diaphyseal populations. Monolayer cultures (P2) were seeded at 1 × 103 cell/cm2, treated with osteogenic and adipogenic (rosiglitazone) medium for 14 days and stained with Alizarin red a and Oil Red O b, respectively. Micromass cultures (2.5 × 105 cells per pellet) were treated with chondrogenic medium for 14 days and stained with Alcian blue c. Error bars are SD. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 (foetal samples, n = 3; 53, 54 and 63 days post conception)

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Stem Cell Research & Therapy

ISSN: 1757-6512