Fig. 6

Inhibition of Oct3/4 attenuated the anti-apoptotic and anti-fibrotic effects of engrafted CSCs in post-MI heart. a Representative images of TUNEL staining (green, TUNEL-positive staining; red, α-actin; blue, DAPI). Scale bar: 100 μm. b Quantitative analysis of TUNEL-positive nuclei. c Representative images of picro-sirius red staining. Scale bar: 50 μm. d Quantitative analysis of interstitial collagen deposition in post-MI hearts. Values shown as mean ± SEM (n = 3–5 per group); &p <0.05 vs. Sham, *p <0.05 vs. MI-PBS, **p <0.01 vs. MI-PBS, #p <0.05 vs. MI + CSCs-Oct3/4(+) control siRNA. CSC cardiac stem cell, DAPI 4,6-diamidino-2-phenylindole, MI myocardial infarction, PBS phosphate-buffered saline, siRNA small interfering RNA, TUNEL terminal deoxynucleotidyltransferase nick-end labeling