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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Synergism of MSC-secreted HGF and VEGF in stabilising endothelial barrier function upon lipopolysaccharide stimulation via the Rac1 pathway

Fig. 6

MSC-secreted VEGF and HGF decreased HPMEC apoptosis and improved cell viability. a, b LPS induced early apoptosis of HPMECs. MSC-CM treatment significantly reduced the number of early apoptotic cells. However, the effect of MSCs was significantly blocked by neutralising HGF or VEGF from the MSC-CM with anti-HGF or anti-VEGF antibody, respectively. c MTT assay confirmed that MSC-CM treatment restored cell viability to a greater extent than LPS stimulation only. Additionally, the effect of MSC-CM treatment was significantly inhibited by neutralising VEGF from the MSC-CM with anti-VEGF antibody. Adding MSC-CM in all groups except control group and LPS group. n = 3, *p < 0.05, **p < 0.01 vs. control group; # p < 0.05 vs. LPS group; &p < 0.05; &&p < 0.01 vs. MSC-CM group. CM Conditioned medium, FITC Fluorescein isothiocyanate, HGF Hepatocyte growth factor, LPS Lipopolysaccharide, MSC Mesenchymal stem cell, VEGF Vascular endothelial growth factor

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Stem Cell Research & Therapy

ISSN: 1757-6512