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Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: Modeling autosomal dominant optic atrophy using induced pluripotent stem cells and identifying potential therapeutic targets

Fig. 9

Addition of 17β-estradiol in RGC differentiation medium promoted generation of OPA1 +/−-RGCs. Control and VO RGCs were incubated with 100 nM 17β-estradiol in the cell culture medium during all of the differentiation stages, including iPSC culturing. Putative RGCs were then fixed with 4 % PFA on day 31. a TUJ1 (green) and BRN3a (red) staining are shown. b TUJ1 (green) and ISLET-1 (red) staining are shown. Scale bars = 50 μM in panel (a) and 100 μM in panel (b)

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Stem Cell Research & Therapy

ISSN: 1757-6512