Table 1 Primer sequences and procedure parameters used in the quantitative PCR analysis

β-actin

5′-GTGGGGCGCCCCAGGCACCA-3′ (forward)

56

42

5′-CTTCCTTAATGTCACGCACGATTTC-3′ (reverse)

Wnt11

5′-TGCAGGAGCTGCAGGATGTGG-3′ (forward)

59

39

5′-AGCTCCATGGAGTGTCTCCAG-3′ (reverse)

OC

5′-ATGAGAGCCCTCACACTCCTC-3′ (forward)

60

28

5′-GCCGTAGAAGCGCCGATAGGC-3 (reverse)

Runx-2

5′-ACGACAACCGCACCATGGT-3′ (forward)

60

28

5′-CTGTAATCTGACTCTGTCCT-3′ (reverse)

ALP

5′-TGGAGCTTCAGAAGCTCAACACCA-3′ (forward)

58

30

5′-ATCTCGTTGTCTGAGTACCAGTCC-3′ (reverse)

Osterix

5′-GCAGCTAGAAGGGAGTGGTG-3′ (forward)

58

42

5′-GCAGGCAGGTGAACTTCTTC-3′ (reverse)

Collagen I

5′-CCTGAGCCAGCAGATTGA-3′ (forward)

59

29

5′-TCCGCTCTTCCAGTCAG-3′ (reverse)

BSP

5′-AAGGCTACGATGGCTATGATGGT-3′ (forward)

61

30

5′-AATGGTAGCCGGATGCAAAG-3′ (reverse)