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Table 1 Primer sequences and procedure parameters used in the quantitative PCR analysis

From: Wnt11 plays an important role in the osteogenesis of human mesenchymal stem cells in a PHA/FN/ALG composite scaffold: possible treatment for infected bone defect

Gene name Primer sequence Annealing temperature (Ta) (°C) Cycles
β-actin 5′-GTGGGGCGCCCCAGGCACCA-3′ (forward) 56 42
  5′-CTTCCTTAATGTCACGCACGATTTC-3′ (reverse)
Wnt11 5′-TGCAGGAGCTGCAGGATGTGG-3′ (forward) 59 39
  5′-AGCTCCATGGAGTGTCTCCAG-3′ (reverse)
OC 5′-ATGAGAGCCCTCACACTCCTC-3′ (forward) 60 28
  5′-GCCGTAGAAGCGCCGATAGGC-3 (reverse)
Runx-2 5′-ACGACAACCGCACCATGGT-3′ (forward) 60 28
  5′-CTGTAATCTGACTCTGTCCT-3′ (reverse)
ALP 5′-TGGAGCTTCAGAAGCTCAACACCA-3′ (forward) 58 30
  5′-ATCTCGTTGTCTGAGTACCAGTCC-3′ (reverse)
Osterix 5′-GCAGCTAGAAGGGAGTGGTG-3′ (forward) 58 42
  5′-GCAGGCAGGTGAACTTCTTC-3′ (reverse)
Collagen I 5′-CCTGAGCCAGCAGATTGA-3′ (forward) 59 29
  5′-TCCGCTCTTCCAGTCAG-3′ (reverse)
BSP 5′-AAGGCTACGATGGCTATGATGGT-3′ (forward) 61 30
  5′-AATGGTAGCCGGATGCAAAG-3′ (reverse)