Fig. 8

Analysis of MSC homing to UC tissues. Coculture of about 100 mg conditioned UC tissue without further MSC outgrowth (n = 4) was performed together with GFP-labeled MSCs seeded at 1000 cells/cm² in comparison with a monoculture of MSC^GFP at the same cell density. After 6 days of coculture, the UC tissue was removed, washed three times in PBS, and documented by fluorescence microscopy. Moreover, the UC tissue and the remaining originally cocultured cells as well as the corresponding monoculture of MSC^GFP were lysed by the addition of SDS and appropriate fluorescence intensities were quantified in aliquots using a Fluoroskan Ascent FL microtiter plate reader and calculated as mean ± SD (n = 4) for the amount of cells according to a standard curve of GFP-labeled MSC. UC umbilical cord