Fig. 4

Histological analyses of lymphangiogenesis. a Quantitative analysis showed significantly decreased footpad thickness in each group. b Fibrosis of the hind limb footpad was detected with H & E stain. c Lymphatic endothelium marker, LYVE-1, was stained brown in HCM groups. Lymphatic vessels are stained brown (arrows). The scale bar represents 100 μm. d Fluorescence imaging of lymphatic vessels on mouse hind limbs after foot pad injection of FITC-dextran. Values are represented as the mean of three measurements with the SD indicated by error bars. *p <0.001, ^# p <0.05. HCM hypoxic conditioned media, LYVE-1 lymphatic vessel endothelial hyaluronan receptor 1, NCM normoxic conditioned media