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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Genome-wide gene expression analyses reveal unique cellular characteristics related to the amenability of HPC/HSCs into high-quality induced pluripotent stem cells

Fig. 4

Schematic illustration of the participation of select tumor suppressor genes (p21, Ink4a, and Arf) and an EMT factor (Snail1) in the amenability of HPC/HSC reprogramming. Select tumor suppressor and mesenchymal genes were downregulated in HPC/HSCs and select oncogenes were upregulated in HPC/HSCs compared with MEFs, indicating that these genes may play important roles in the reprogramming of HPC/HSCs. The downregulation of tumor suppressor genes (p21, Ink4a, and Arf) and Snail1 in HPC/HSCs triggers the amenability of HPC/HSCs to OSKM-mediated reprogramming. Independent ectopic activation of p21, Ink4a, Arf, and Snail1 along with OSKM decreases the efficiency of HPC/HSC reprogramming. Dox doxycycline, HPC/HSC hematopoietic progenitor and stem cell, IL interleukin, MEF mouse embryonic fibroblast, 4N tetraploid complementation, OSKM Oct4, Sox2, Klf4, and c-Myc, SCF stem cell factor

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