Fig. 3
Mesenchymal stromal cell (MSC) administration prevents retinal ganglion cell (RGC) loss. Twelve weeks after STZ administration, diabetic mice received an intravitreal injection of 2 μL saline (DM) or 2 × 105 MSCs resuspended in 2 μL saline (DM + MSC). Retinal histology was analyzed in serial 4-μm eye sections, immunolabeled with β3-tubulin antibody (marker of RGCs). Samples were observed under confocal microscopy focusing on the ganglion cell layer of the retina (arrows), a 4 weeks and b 12 weeks after MSC administration. RGCs in the ganglion cell layer of the retina were quantified at both experimental times (c and d). Qualitative data are representative of eight eyes per group and per analyzed time. Quantitative data correspond to mean ± SEM of eight eyes per group and per analyzed time