Fig. 4

Expression of eNOS and NO production in transfected HEK293T cells. a Fluorescence microscopy of transfected HEK293T cells with P-eNOS and MC-eNOS with 0.5 μg plasmid DNA. b Detection of eNOS protein expression in transfected HEK293T by western blot analysis. c NO production in HEK293T cells at 24 h and 48 h post-transfection with P-eNOS and MC-eNOS plasmids using the griess assay, and d detection of nitric oxide production in living cells following P-eNOS and MC-eNOS transfection and non-transfected control by DAF-2 fluorescence. *p < 0.05 and **p < 0.05 vs. MC-GFP, P-GFP, and HEK293T. MC-eNOS minicircle vector expressing endothelial nitric oxide synthase, MC-GFP minicircle vector expressing green fluorescent protein, P-eNOS plasmid vector expressing endothelial nitric oxide synthase, P-GFP plasmid vector expressing green fluorescent protein