Fig. 1
From: Lin28A induces energetic switching to glycolytic metabolism in human embryonic kidney cells
Characterisation of Lin28A over-expression in HEK 293 cells. Real-time PCR revealed that Lin28 mRNA expression levels were increased in Lin28A over-expressing cells, n = 3 (a). Conversely, Let 7 a, c and g miRNA expression levels were decreased in Lin28 over-expressing cells, n = 3 (b). Western blot analysis (c) confirmed that Lin28 protein levels were also increased in over-expressing HEK cells (d), n = 4. GAPDH and U6 were used as housekeeping genes for mRNA and miRNA expression, respectively. Student’s unpaired t-tests were used to compare between experimental groups. Data are represented as mean ± SEM. HEK human embryonic kidney, miRNA microRNA