Fig. 2

Lin28A promotes energetic switching to a more glycolytic phenotype. Extracellular flux analysis using the Seahorse XF24 bio-analyser demonstrated that ECAR was significantly increased in Lin28A over-expressing cells after addition of 10 mM glucose (glycolysis). ECAR was also increased after subsequent addition of 1 μM oligomycin (glycolytic capacity) and 100 mM 2-DG (glycolytic reserve) (a and b). Conversely, high resolution oxygen respirometry showed that even without stimulation basal oxygen consumption was reduced in over-expressing cells. No changes in maximal respiration (after addition of 1 μM CCCP) or spare capacity (after addition of 1 μM antimycin and 1 μM rotenone) were observed (c). Student’s unpaired t-tests were used to compare between experimental groups. Data are represented as mean ± SEM, n = 5 for all data points. ECAR extracellular acidification rate, 2-DG 2-deoxyglucose, CCCP carbonyl cyanide m-chlorophenyl hydrazone