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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Relevance of HCN2-expressing human mesenchymal stem cells for the generation of biological pacemakers

Fig. 7

Intercellular coupling of cells growing on the opposite sides of Kapton® scaffold. a DAPI (20 μM) was injected through the patch pipette into the mHCN2-transfected cell 1 (nucleus encircled in c) residing on the top side of Kapton® scaffold. b Non-transfected hMSCs (cell 2 and cell 3) residing on the bottom side of the scaffold (nucleus encircled in c). c Transfer of DAPI dye from the donor cell 1 to the recipient cell 2 and cell 3. Note that the fluorescence of DAPI-stained nucleus in cell 1 could not be demonstrated because of the impermeability of Kapton® scaffold to UV light. d DAPI accumulation kinetics measured in the regions of interest (ROI) situated on the nuclei of respective cells. Arrow indicates the onset of dye application to cell 1

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