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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: EDTA soluble chemical components and the conditioned medium from mobilized dental pulp stem cells contain an inductive microenvironment, promoting cell proliferation, migration, and odontoblastic differentiation

Fig. 3

Pulp regeneration after transplantation of the autoclaved teeth reconstituted with soluble extracts. Twenty-eight days after ectopic transplantation of MDPSCs (a, g, n, u) in the native autoclaved teeth only, and in the autoclaved teeth reconstituted (b, h, o, v) with the GdnHCl extracts, (c, i, p, w) with the EDTA extracts, (d, j, q, x) with the MDPSC CM, (e, k, r, y) with the GdnHCl extracts and the CM, and (f, l, s, z) with the EDTA extracts and the CM. a–l H & E staining. Pulp-like cells (arrows). m Ratio of regenerated area to root canal area. n–s Hoechst 33342 staining. t Ratio of Hoechst 33342-positive cells to regenerated area. u–z Immunostaining with RECA1. zz Ratio of vascularization area to the total regenerated area. Data are expressed as means ± SD of four determinations. *P < 0.05, **P < 0.01, # P < 0.001 to nonextracted tooth, reconstituted tooth with the MDPSC CM alone, and MDPSC CM with the GdnHCl extracts or the EDTA extracts. CM conditioned medium, RECA1 rat endothelial cell antigen 1

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Stem Cell Research & Therapy

ISSN: 1757-6512