Fig. 5
From: Sphere-forming cells from peripheral cornea demonstrate the ability to repopulate the ocular surface
Peripheral corneal spheres implanted into corneosceral tissue repopulate the ocular surface. LIVE/DEAD® staining (green) of implanted spheres, 5× magnification, at 0 h post implantation a and 4 days post implantation b show cell migration from the spheres appearing as green streaks out from the sphere. At 7 days post implantation c, the entire corneal bed appears repopulated with live cells. Representative cells at the leading migratory edge of the corneal surface d and cells on the corneal surface (taken from the region indicated by * in c) e show differing morphology. Representative cells over the limbal region f and sclera g (taken from the regions indicated by ** and *** in c respectively) show a different cell migration pattern and morphology to that observed in the corneal tissue. Scale bar = 1000 μm for a, b, e and 100 μm for c, d, f, g (Colour figure online)