Fig. 4
PMA enhances p27 expression in hMSCs. a hMSCs were treated with PMA (1 μM) for 12 h. The expression of p27kip1 (p27) mRNA was measured by RT-RCR. b The p27 protein expression level was examined by Western blotting. The cells were treated with 1 μM PMA for up to 12 h. c PCNA expression was detected by Western blotting. d Schematic drawings of PMA-activated signaling pathways that lead to NFAT activation. *p < 0.05, **p < 0.01, versus control. GSK3β glycogen synthase kinase 3 beta, MARCKS myristoylated alanine-rich C kinase substrate, NFAT nuclear factor of activated T cells, PCNA proliferating cell nuclear antigen, PKC protein kinase C, PMA phorbol 12-myristate 13-acetate