Fig. 3

iHepL cells express hepatic genes. a Representative fluorescence images of HepG2, mouse embryonic fibroblasts (MEF), and hepatic progenitor-like (iHepL) cells immunostained with antibodies against multiple proteins. No differences were observed between the iHepL pool or clones 2, 6, and 15 (Additional file 1: Figure S3). Nuclei were stained with DAPI. b Representative fluorescence images at low magnification of MEF and iHepL cells immunostained for Albumin (Alb), Ugt1a1, Glucokinase (Gck), and Glycogen synthase 2 (Gys2). Nuclei were stained with DAPI. Bar diagrams in the right panel show the signal intensity distribution (strong, medium, or weak) in MEF or iHepL cells by the high content screening imaging station Scan® from Olympus. Data are represented as mean ± SD. Student’s t test was performed between iHepL and MEF. and statistical significance was p < 0.01 in all cases