Fig. 5

Repopulation of the liver of hepatectomized mouse after hepatic irradiation. Approximately 106 iHepL cells (GFP-positive) were transplanted into partial hepatectomized and hepatic irradiated mice (n = 5). a Low magnification representative fluorescent images showing engraftment of iHepL cells in the mouse liver after 3 weeks. b Co-immunofluorescence staining of GFP with several hepatocyte and cholangiocyte markers. Nuclei were counterstained with DAPI (blue). Red blood cell auto-fluorescence is observed in some images. GFP green fluorescent protein