Fig. 4

ADSC-CM promoted wound healing and reduced scar formation. a, b The morphology of incisions were observed at different time points after injecting subcutaneously with DMEM and 80 % concentration of ADSC-CM. Relative wound areas are shown by histogram. c, d The histopathological change of wound healing was shown by H&E staining between DMEM and 80 % concentration of ADSC-CM group. The histologic scores were assessed by histogram. Scale bars = 400 μm. e Masson’s trichrome staining labeled collagen in wound healing tissues of mice treated with DMEM and 80 % concentration of ADSC-CM. f–h Immunohistochemistry staining of Col1 and Col3 in wound healing tissues of mice treated with DMEM and 80 % concentration of ADSC-CM. Relative density of collagen was shown by histogram. Scale bars = 50 μm (n = 6 mice per group). ADSC-CM adipose tissue-derived stem cell-conditioned medium, Col1 collagen I, Col3 collagen III, D days, DMEM Dulbecco’s modified Eagle medium