Fig. 8

Reduction of CD90 expression stimulates the adipogenesis of mesenchymal stromal cells (MSCs). MSCs, short hairpin (sh)RNA control MSCs, shRNA CD90 MSCs, and CD90-negative MSCs were tested for their ability to differentiate into adipogenic lineages. a Representative photomicrograph images show oil red staining indicative of adipogenic differentiation. MSCs from dental pulp (DPSC), amniotic fluid (AF-MSCs), and lipoaspirate (ADSC) were cultured in the non-differentiation medium MSCs (control) and adipogenic differentiation medium (AD). The images shown are representative of two independent experiments. b Oil red dye retained in the lipid vacuoles was measured by determining the optical density (OD) at 510 nm. Data shown represent the mean ± SD of one experiment performed in triplicate (n = 7). *p < 0.05; **p < 0.01; ***p < 0.001