Fig. 3

ES-CM inhibited Stat3 signaling pathway in 4T1 cells. a Rluc imaging of activated Stat3 in vitro controlled by 4T1-CM and DMEM. b Quantitative analysis of imaging signals. The signal activity showed the suppressed effect of ES-CM group. **P < 0.01 vs 4T1-CM, ^## P < 0.01 vs DMEM, ***P < 0.001 vs 4T1-CM, ^### P < 0.001 vs DMEM, n = 3. c PCR analysis of Stat3 signaling pathway-related gene expression. **P < 0.01 vs 4T1-CM, ^## P < 0.01 vs DMEM, n = 3. d ES-CM decreased the phosphorylation of Stat3 in 4T1 cells. Representative western blots showing the level of pStat3 and total Stat3. Data are presented as mean ± SD. **P < 0.01 vs 4T1-CM, ^## P < 0.01 vs DMEM, n = 3. See also Additional file 1: Figure S3. CM conditioned medium, DMEM Dulbecco’s modified Eagle medium, ES embryonic stem