Fig. 7

ES-CM weakened 4T1 cancer cell stemness. a Real-time RT-PCR analysis of stemness-related gene expression of 4T1 cells in each group treated with CMs for 48 h. **P < 0.01 vs 4T1-CM, ^## P < 0.01 vs DMEM, n = 3. b 4T1 cells after treatment with 4T1-CM and ES-CM for 48 h respectively were plated in low-density suspension cultures for sphere formation 4 days after plating. Experiment performed in triplicate. Data presented as mean ± SD. **P < 0.01 vs 4T1-CM. c–f Protein expression level of β-catenin c, Sox2 d, Oct4 e, and Nanog f in 4T1 cells examined by western blot. *P < 0.05 vs 4T1-CM, ^# P < 0.05 vs DMEM, **P < 0.01 vs 4T1-CM, ^## P < 0.01 vs DMEM, ***P < 0.001 vs 4T1-CM, ^### P < 0.001 vs DMEM, n = 3. CM conditioned medium, DMEM Dulbecco’s modified Eagle medium, ES embryonic stem, MMP matrix metalloproteinase