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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production

Fig. 3

Validation of nine non-classical markers by flow cytometry among 15 clinical-grade adipose-derived mesenchymal stromal cells (AMSCs). Expression of five classical (a) and nine non-classical markers (b) was validated by flow cytometry across 15 additional freshly isolated and expanded AMSC donors. Surface marker expression was evaluated by the percentage of the gated cell population and mean fluorescence intensity (MFI). c Representative histograms of surface marker expression compared to unstained AMSCs (negative control). Of the non-classical markers, CD36 exhibited two cell populations which varied from patient-to-patient

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Stem Cell Research & Therapy

ISSN: 1757-6512