Fig. 3

Senescence of irradiated ASCs. a Crystal violet staining. Cell morphology was inhomogeneous, smaller, and blunter in the 6R group compared to the other groups. b β-galactosidase immunostaining. Positive β-galactosidase staining (arrowhead) was rarely observed in the N groups, but was readily apparent in the 6R group. c Immunophenotyping by flow cytometry. All groups demonstrated reduced expression of the hematopoietic surface markers CD 31 and CD41. The 6R groups demonstrated lower CD90 expression than the other groups