Fig. 3
From: A strategy to ensure safety of stem cell-derived retinal pigment epithelium cells
Sorting for CD59 for RPE purification and stem cell removal. a Use of flow cytometry-based sorting to collect the population expressing CD59 (CD59+) separately from the population not expressing CD59 (CD59–) in a 1:1 mixture of hESCs and RPE cells. b Representative image showing pigmentation in the cell pellets obtained from the CD59+ and CD59– fractions. c Quantitative PCR to measure expression of pluripotency (top) and RPE markers (bottom) in CD59+ and CD59– fractions. The pre-sorted cell suspension is used for comparison. ACT and GAPDH were used as housekeeping genes (n = 4, ± SD). d Representative bright-field images showing the cobblestone architecture of CD59+ and CD59– fractions seeded at a density of 78,000 cells/cm2 and cultured for a period of 45 days post sorting. Scale bar = 200 μm. e No CD59-PE fluorescence can be seen in cells described in d as compared with cells freshly stained for CD59 used as a positive control (CD59 pos. ctrl)