Fig. 1

Characterization of human placenta-derived MSCs and De-MSCs. a Cell morphology of MSCs (×25), Ob-MSCs (×25), De-MSCs (×25) and Re-MSCs (×25). b The staining of Alizarin Red S, Oil Red O and Toluidine Blue respectively for osteogenesis (×25), adipogenesis (×400) and chondrogenesis (×400) of MSCs and De-MSCs. c Flow cytometry (FCM) analysis of the expression of indicated cell surface markers in MSCs, Ob-MSCs, De-MSCs and Re-MSCs. d Positive rates of FCM presented in the histogram were mean ± SD of three independent experiments. One-way ANOVA test was employed for intergroup comparison. ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001. De-MSCs dedifferentiated MSCs, MSCs mesenchymal stem cells, Ob-MSCs osteoblasts differentiated from MSCs, Re-MSCs osteoblasts differentiated from De-MSCs