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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Improved osteogenesis and upregulated immunogenicity in human placenta-derived mesenchymal stem cells primed with osteogenic induction medium

Fig. 1

Characterization of human placenta-derived MSCs and De-MSCs. a Cell morphology of MSCs (×25), Ob-MSCs (×25), De-MSCs (×25) and Re-MSCs (×25). b The staining of Alizarin Red S, Oil Red O and Toluidine Blue respectively for osteogenesis (×25), adipogenesis (×400) and chondrogenesis (×400) of MSCs and De-MSCs. c Flow cytometry (FCM) analysis of the expression of indicated cell surface markers in MSCs, Ob-MSCs, De-MSCs and Re-MSCs. d Positive rates of FCM presented in the histogram were mean ± SD of three independent experiments. One-way ANOVA test was employed for intergroup comparison. * P < 0.05, ** P < 0.01, *** P < 0.001. De-MSCs dedifferentiated MSCs, MSCs mesenchymal stem cells, Ob-MSCs osteoblasts differentiated from MSCs, Re-MSCs osteoblasts differentiated from De-MSCs

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