Fig. 4
The immunogenic properties of MSCs and De-MSCs in vitro. a–bCo-stimulatory molecules were detected by FCM. Histograms in black showed isotype control staining and histograms in red showed the specific expression of the indicated cells. Values of positive rate presented in the histogram were mean ± SD of three independent experiments. c CD3+ T cells or activated CD3+ T cells were cultured with MMC-treated MSCs, Ob-MSCs, De-MSCs and Re-MSCs in 96-well plates for 72 h. The proliferation of T cells was assayed by tritiated thymidine ([3H]TdR) incorporation. Values of cpm presented were mean ± SD. T T cells, aT T cells activated by anti-CD3 and anti-CD28, aT + M activated T cells co-cultured with MSCs, aT + Ob activated T cells co-cultured with Ob-MSCs, aT + De-M activated T cells co-cultured with De-MSCs, aT + Re-ob activated T cells co-cultured with Re-MSCs (n = 3/group). * P < 0.05, ** P < 0.01. One-way ANOVA test was employed for intergroup comparison