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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Preclinical study of mouse pluripotent parthenogenetic embryonic stem cell derivatives for the construction of tissue-engineered skin equivalent

Fig. 3

Multilineage differentiation and characteristics analysis of MSCs. a Schematic outline of pESC and ESC induction and fibroblastic differentiation flow chart. b Flow cytometry analysis of pMSCs and eMSCs indicates that these cells express MSC surface antigens. c Morphological appearance of pMSCs and eMSCs before osteogenic, chondrogenic, and adipogenic differentiation. Von Kossa and Alizarin Red S staining 21 days after osteogenic induction. Safranin O staining 21 days after chondrogenic induction. Oil Red O staining 14 days after adipogenic induction. Bars = 100 μm. d RT-PCR analysis of the gene expression profiles related to osteogenic, chondrogenic, and adipogenic differentiation. EB embryoid body, eMSC MSC derived from ESC, ESC embryonic stem cell, MSC mesenchymal stem cell, pESC parthenogenetic embryonic stem cell, pMSC MSC derived from pESC

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