Table 1 Primers of three germ layer genes and the reference gene for quantitative real-time PCR
Gene | Primers (5′–3′) | Product (bp) |
|---|---|---|
Snai1 | GACCTGTGGAAAGGCCTTCTCTAGG | 170 |
CCTGGCACTGGTATCTCTTCACATC | ||
Hand1 | GCTACGCACATCATCACCATCATC | 125 |
CAGCAGCCAGCTCTGGAAGTAAG | ||
Gata2 | GCCAAAAGAGAGACTGGAGGAAGGG | 82 |
ACACCTCCCACCTTTTAGTCACTCTG | ||
nestin | GTTACCAAAGCCTCTTAGAAATGACC | 577 |
CAGATGCAACTCTGCCTTATCCTC | ||
Oct3/4 | GTGTGAGGTGGAGTCTGGAG | 182 |
AGCCTCATACTCTTCTCGTTGG | ||
Afp | CTCTGGCGATGGGTGTTTAG | 175 |
TGCCTGGAGGTTTCGGGATT | ||
Gapdh | GGTGAAGGTCGGTGTGAACG | 152 |
CTCGCTCCTGGAAGATGGTG |