Fig. 1

Characterization of hADSCs. a Immunofluorescent assay indicated that ADSCs were positive for cell surface markers CD44, CD29, CD105, and CD90 and were negative for CD133, CD34, and CD36. Scale bar, 50 μm. b Flow cytometry also revealed that CD29, CD44, CD90, and CD105 were highly expressed and CD133, CD34, and CD36 were negligible for the cells. c Triplet differentiation assays revealed that ADSCs could differentiate into adipocytes (a), osteocytes (b), and chondrocytes (c), which were stained by Oil Red O, Alizarin red, and Alcian blue, respectively. Scale bar, 25 μm