Fig. 2
From: Ex-vivo expansion of nonhuman primate CD34+ cells by stem cell factor Sall4B
Quality control of vectors and GFP expression in 293 T cells. a Four plasmids (psPAX2, pMD2.G, GFP-control expression vector, and GFP-Sall4B expression vector) were amplified by bacteria amplification system and linearized by single digestion. The restriction endonuclease recognition sites were Sac I, Not I, EcoR I, and EcoR I. b 293 T cells were monitored after 12 hours during the lentivirus packaging process (10 ×). c GFP expression in 293 T cells 12 hours after lentivirus transduction (20 ×)