Fig. 2

Mesenchymal features in P311 WT and P311 KO mouse burn wounds. a Vimentin expression in the proximal and distal neo-epidermis of P311 WT and P311 KO mice was analyzed using immunohistochemistry. Proximal neo-epidermis was defined as the tongue of the neo-epidermis, and distal neo-epidermis was defined as the epidermis at the margin of the wound. Scale bar, 25 μm. b mRNA levels of vimentin, TGFβ1, Snail2, and Twist1 were detected using real-time PCR in P311 WT and P311 KO mouse burns and normal skin samples. c Western blot analysis demonstrating the protein levels of β1-integrin, vimentin, E-cadherin, and active and LAP-bound TGFβ1 in P311 WT and P311 KO mouse burns and normal skin samples. d Relative densities of the target proteins were standardized to the expression of GAPDH. Data are shown as the mean ± SEM, Student’s t test; *P < 0.05 and **P < 0.01 for P311 WT vs P311 KO; #P < 0.05 and ##P < 0.01 for normal skin vs burned wounds