Fig. 7
Hypoxia increases secretion of PlGF in ECFCs a Conditioned medium from ECFCs grown for 48 h at 21% O2 (empty bars) or 1% O2 (filled bars) was incubated on a human angiogenesis Proteome Profiler array to identify soluble factors secreted. Red arrow, position of PlGF on the graph. b, c Signal intensity measured using ImageJ software, and a positive control (Ref) was used to normalise the intensity of PlGF between conditions. d Conditioned medium from ECFCs grown in 21 and 1% O2 was probed using a PlGF-specific ELISA. PlGF concentrations were extrapolated from a standard curve and plotted as mean ± SD. *p < 0.05. e ECFCs were grown in 1% O2 for 11 days. Conditioned medium was harvested and PlGF levels were measured by ELISA. Data plotted as mean ± SD. **p < 0.01 (n = 3). PlGF placental growth factor