Fig. 8
HIF-1α stabilisation induces PlGF expression and alters ECFC tube formation and viability. a ECFCs were grown in Matrigel® and exposed to vehicle (DMSO, VEH) or DMOG (1 mM) for 24 h. Protein lysates were probed for HIF1α (upper panel) or β-actin (lower panel). b PlGF concentration was measured by ELISA in conditioned medium from ECFCs treated with vehicle (DMSO) or DMOG (1 mM) for 24 h. c Conditioned medium (CM)from ECFCs treated with vehicle (DMSO) or DMOG (1 mM) was used to treat ECFCs grown in Matrigel® and stained with PKH for 24 h. Total tube area (μm2) quantified using NIS Elements software. d ECFCs were treated with conditioned medium from vehicle (DMSO) or DMOG (1 mM)-treated cells for 24 h. Cell viability measured using a CCK-8 cell viability assay (Sigma), and plotted as mean % viability ± SD (n = 4). DMOG dimethyloxalylglycine, PlGF placental growth factor. Data plotted as Mean ± SD **p < 0.01; ***p < 0.001