Fig. 4
Frequency of repertoires based on co-expression of six selected CD markers as a function of in vitro expansion. Sorted subpopulations SP1–4 and the control population CFSC sorted on the forward scatter were analyzed at the end of passages two and three (P2, P3). All possible repertoires are shown at P0. Only co-expression variants for CD34, CD146, and CD271 are shown at P2 and P3, since the CD73, CD90, and CD105 markers were invariably co-expressed. The shaded area at P0 at sorting denotes profiles associated with ASC hallmark markers, and the frequencies are also highlighted with individual bars. The legend indicates full profiles of the originally sorted repertoires, and these are highlighted in the plots by a stippled line. The data are presented as means from two independent experiments. ■The marker is expressed. □ The marker is not expressed. Abbreviations: N.A. not applicable