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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mesenchymal stem cells/multipotent stromal cells (MSCs) are glycolytic and thus glucose is a limiting factor of in vitro models of MSC starvation

Fig. 2

Glucose metabolism in MSC cultures. Immortalized MSCs in culture were subjected to Seahorse analyses to monitor glucose utilization via oxygen consumption rate (OCR) (a) as an assessment of oxidative phosphorylation and extracellular acidification (ECAR) (b) as an assessment of glycolysis (additions were 10 μM oligomycin at A, 3000 nM carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) at B, 1000 mM 2-deoxy-d-glucose (2DG) at C, and 10 μM rotenone at D; see Additional file 3: Figure S3 for a further description of interventions). Cycloheximide (CHX) treatments were included as a baseline non-respiratory control. Intracellular ATP in response to 2-h treatments was also assessed (c). Results showed repeatable trends over three separate studies. N = 4. EGF-Epidermal Growth Factor, FasL-Fas Ligand, txt-Treatment

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