Fig. 4
From: High glucose suppresses embryonic stem cell differentiation into cardiomyocytes
High glucose suppresses cardiomyocyte-specific marker expression. a mRNA levels of cardiomyocyte structure proteins, MEF2C, and TNNT2. b Representative images of TNNT2 immunofluorescent staining. c Quantitative fluorescent density data of TNNT2 immunofluorescent staining. d MEF2C and TNNT2 protein expression at differentiation day 9. Gene expression was assessed at EB formation day 5 and differentiation day 1, 3, 5, and 7. Experiments were repeated three times (n = 3). EB embryoid body derived from E14 or GR-E14 cells, HG high glucose (25 mM), LG low glucose (5 mM). Values were dedicated as mean ± SD. *Indicates significant difference compared with the other group