Fig. 3

Neural retinal differentiation potential of eye-wall c-kit⁺/SSEA1⁻ progenitor cells. The cells were cultured in differentiation media for 8–10 days and were stained with markers for neurons or Müller cells and with DAPI for counting nuclei (blue). Representative images showing cells positive for Recoverin (Rec; A), Rhodopsin (Rho; B), protein kinase C alpha (PKCα; C), Calbindin (Calb; D), glutamate decarboxylase 65 & 67 (GAD; E), choline acetyltransferase (ChAT; F), glutamine synthetase (GS; G), and glial fibrillary acidic protein (GFAP; H). Areas in the white boxes in A and B are shown at higher magnification in A1 and B1, respectively. Cells were harvested after differentiation for 8–10 days and were stained for markers of neurons and Müller cells, as shown in the FITC and PE channels. Representative flow cytometry plots showing the percentages of cells positive for Rec (27.6%; A′), Rho (12.5%; B′), PKCα (16.5%; C′), Calb (35.3%; D′), GAD (17.1%; G′), ChAT (29.1%; F′), GS (31%; G′), and GFAP (15.1%; H′). DAPI 4′,6-diamidino-2-phenylindole. Scale bars represent 50 μm for all images (Color figure online)