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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Grafted c-kit+/SSEA1− eye-wall progenitor cells delay retinal degeneration in mice by regulating neural plasticity and forming new graft-to-host synapses

Fig. 4

Transdifferentiation capability of eye-wall c-kit+/SSEA1− progenitor cells. (A) Day 1 in which the medium of c-kit+/SSEA1− cells was switched to the RPE differentiation medium. (B) Pigment (arrowhead) appeared after 4–8 weeks. (C) Pigment (arrow) could be seen in the dish. Representative immunostaining images showing cells positive for paired box protein 6 (Pax6; D), microphthalmia-associated transcription factor (MITF; E), Calponin (F), and von Willebrand factor (vWF; G). Differentiated cells were stained for markers shown in the FITC and APC channels. Representative flow cytometry plots showing the percentages of cells positive for Pax6 (26.6%; D′), MITF (16.9%; E′), Calponin (27.3%; F′), and vWF (25.6%; G′). DAPI 4′,6-diamidino-2-phenylindole. Scale bars represent 50 μm for all the images (Color figure online)

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Stem Cell Research & Therapy

ISSN: 1757-6512