Fig. 3

Cardiogenic differentiation in the different cell groups. Cardiogenic differentiation of MSCs in the different cell groups was evaluated by the expressions of cTnT and α-SA. A Confocal microscopy of immunofluorescent staining of DAPI-labeled MSCs induced by 5-AZA after 14 days (200×). Cells stained with antibody to cTnT appeared green, and cells stained with antibody to α-SA appeared red. A1–A3 represented the expressions of cTnT and α-SA under normoxic condition, and A4–A6 represented the expressions of cTnT and α-SA under the hypoxia/reoxygenation (HR) condition; A1 and A4, A2 and A5, and A3 and A6 represented the blank control group, null vector control group, and lncRNA-Bvht group respectively. a Cells derived from DAPI-labeled MSCs induced by 5-AZA displayed blue nuclei; (b) Cells stained with antibody to cTnT appeared green in A1-A6; (c) Cells stained with antibody to α-SA appeared red in A1-A6; (d) Merged image of a, b, and c. B Comparison of the percentage of cTnT-positive cells among the different groups under normoxic condition and after hypoxia/reoxygenation respectively. C Western blot (a) and quantitative real-time PCR (b) analysis of the expressions of cTnT and α-SA in the different cell groups. **P <0.01 , versus blank control; ^## P <0.01 , versus null vector control