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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Optimizing mesoderm progenitor selection and three-dimensional microniche culture allows highly efficient endothelial differentiation and ischemic tissue repair from human pluripotent stem cells

Fig. 3

S1P greatly enhanced CD31 expression and upregulated endothelial marker genes expression. a Protocol for endothelial differentiation. b MESP1+ cells have significantly greater ability to differentiate to endothelial cells compared with MESP1– cells. c Q-PCR analysis of S1P receptor expression in undifferentiated hESC, differentiation day 3 MESP1-mTomato– (MESP1–) and MESP1-mTomato+ cells (MESP1+). d Immunostaining analysis showing that S1P treatment greatly enhanced the generation of CD31+ cells on differentiation day 8. Scale bars: 100 μm. e Quantification of FACS analysis of CD31+ cells percentage after S1P treatment on differentiation day 8 (n = 3, **** p < 0.0001). f Q-PCR analysis showing S1P treatment significantly upregulated endothelial marker genes expression (n = 3)

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Stem Cell Research & Therapy

ISSN: 1757-6512