Fig. 1

The adipose-derived stem cell cultured supernatant (ASC sup) reduced OVA-Alum-induced allergic airway inflammation. a Mice were sensitized on days 0, 1, 7, and 8 by intraperitoneal injection of OVA plus Alum or PBS (control) only. The mice were challenged on days 14, 15, 21, and 22 intranasally with OVA. 10 μg/50 μL of the con sup or ASC sup were injected intranasally on days 12, 13, 19, and 20. b The enhanced pause (PenH) was evaluated at baseline and after treatment with increasing doses of aerosolized methacholine (0–50 mg/ml). Open triangle; PBS-treated mice [PBS], open circle; Ova-Alum-treated mice [OVA], solid triangle; control sup-treated mice after Ova-Alum treatment [con sup + OVA], solid square; ASC sup-treated mice after Ova-Alum treatment [ASC sup + OVA]. b Airway resistance values in response to methacholine (0 to 50 mg/ml) were evaluated. c The number of inflammatory cells in the BALF samples was counted after Diff-Quik staining. (^* p < 0.05, ^*** p < 0.001, n = 5 mice per group, experiments were performed in triplicate)