Fig. 3

Treating with ASC sup regulated cytokine expression levels in OVA-induced BALF and LLNs. Cytokine concentrations in BALF(a) and in the culture medium of CD3-stimulated lymphocytes isolated from LLNs(b). For activation of lymphocytes from LLN, the wells were incubated with 1 μg/ml of anti-CD3 antibody for 16 hours at 4 °C. The lymphocytes (4 × 10⁵ cells per well) were introduced to the well and incubated for 3 days. After activation, the levels of several cytokines were measured in the supernatant using ELISA kits; ELISA assays were conducted in accordance with the manufacturer’s instructions. (PBS; BALF or culture supernatant of LLN from PBS-treated mice, OVA; BALF or culture supernatant of LLN from OVA-Alum-induced airway inflammation mice, con sup + OVA; BALF or culture supernatant of LLN from OVA-Alum-induced airway inflammation mice with con sup treatment, ASC sup + OVA; BALF or culture supernatant of LLN from OVA-Alum-induced airway inflammation mice with ASC sup treatment. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, n = 5 mice per group, experiments were performed in triplicate)