Fig. 5
From: Phenotype and multipotency of rabbit (Oryctolagus cuniculus) amniotic stem cells
Differentiation assays (adipogenic, osteogenic and chondrogenic lineages) of cells isolated from the rabbit amniotic membrane. a, b Adipogenic differentiation, as shown by Oil Red staining. Lipid vesicles in the cytoplasm of cells (arrows). In addition, the nucleus had moved to the periphery of the cell. c, d Osteogenic differentiation as shown by Von Kossa and Alizarin Red staining, respectively. Note the presence of calcified regions (arrows) in the extracellular matrix (EM). e, f Chondrogenic differentiation, as shown by Masson’s Trichrome and Picrosirius staining, respectively. Cells showed a rounded shape with a globular nucleus (N) more strongly stained by Masson’s Trichrome which were surround by an extensive matrix rich in collagen fibres (CF), similar to the morphology of chondroblasts. In addition, there was formation of chondrocyte lacunae (CL)