Fig. 6
Metformin (MET) treatment exerted an anti-apoptotic effect on CDCs subjected to oxidative stress by activating AMPK. a1 Annexin V-PI double staining assay of CDCs treated with H2O2, analyzed by flow cytometric analysis. a2 Average percentages of apoptotic CDCs. n = 9. b Representative images (b1) and quantification (b2) of TUNEL staining in CDCs subjected to H2O2 treatment. Cell nuclei were stained with DAPI (blue) and TUNEL+ nuclei were labeled with TMR (green). TUNEL positive rate = (TUNEL-positive nuclei/DAPI-positive nuclei) × 100%. n = 12. Scale bar = 30 μm. c Cell viability of CDCs detected with CCK8 analysis. The data were normalized to 100% of the control group. Data were analyzed by one-way ANOVA with post-hoc comparisons by the Tukey’s test. *P < 0.05 vs. control; # P < 0.05 vs. H2O2. CC compound C