Fig. 3
Renal cell proliferation in IRI-mice treated with different stem/progenitor cells and their derived EVs. a Quantification of PCNA-positive cells/high power field (HPF: ×40) was performed in renal sections of IRI mice injected with vehicle alone (IRI-CTL), 1 × 105 Gl-MSCs (IRI-Gl-MSC), 400 × 106 EVs from Gl-MSCs (IRI-Gl-MSC-EV), 400 × 106 EVs from Gl-MSCs purified by floating process (IRI-Gl-MSC-EV-float), 400 × 106 EVs from Gl-MSCs and treated with RNase (IRI-RNase-Gl-MSC-EV), 1 × 105 T-CD133+ cells (IRI-T-CD133 +), 480 × 106 EVs produced by T-CD133+ cells (IRI-T-CD133 + -EV), 230 × 106 EVs derived from fibroblasts (IRI-F-EV), and in sham-operated SCID mice. ANOVA with Dunnett’s multiple comparison test was performed, (* p < 0.05). b Representative micrographs of PCNA staining preformed on section of kidneys of IRI-AKI mice injected with different treatments. Original magnification: ×40