The effects of glomerular and tubular renal progenitors and derived extracellular vesicles on recovery from acute kidney injury

Table 2 Schematic representation of the groups of mice used in the study

	Treatment	Doses	No. animals	Time of sacrifice
	Normal	-	5	48 h
	Normal	-	5	3 weeks
	Sham-operated	-	5	48 h
	IRI-CTL (vehicle)	-	8	48 h
	IRI-CTL (vehicle)	-	6	3 weeks
Cells	IRI-Gl-MSC	10⁵ cells	8	48 h
	IRI-Gl-MSC	10⁵ cells	6	3 weeks
	IRI-T-CD133⁺	10⁵ cells	8	48 h
	IRI-T-CD133⁺	10⁵ cells	6	3 weeks
EVs	IRI-Gl-MSC-EVs	400 × 10⁶ EVs^*	8	48 h
	IRI-Gl-MSC-EVs-float	400 × 10⁶ EVs^*	6	48 h
	IRI-RNase-Gl-MSC-EVs	400 × 10⁶ EVs^*	8	48 h
	IRI-T-CD133⁺-EVs	480 × 10⁶ EVs^*	8	48 h
	IRI-F-EVs	230 × 10⁶ EVs^*	5	48 h

IRI ischemia-reperfusion injury, CTL control, Gl-MSC glomerular mesenchymal stromal cells, T-CD133 ⁺ -EVs T-CD133⁺-derived EVs, EVs extracellular vesicles, Gl-MSC-EVs Gl-MSC-derived EVs, Gl-MSC-EVs-float Gl-MSC-derived EVs isolated through floating test, RNase-Gl-MSC-EVs Gl-MSC-derived EVs treated with 5 μg/ml RNase, T-CD133 ⁺ CD133⁺ renal tubular cells, F-EVs fibroblast-derived EVs. *The doses of EVs used were selected as the number of EVs produced overnight by 10⁵ cells in serum starvation

ISSN: 1757-6512