Fig. 4

XR_111050 enhanced the osteogenic differentiation of BMSCs. The BMSCs were transduced with XR_111050 lentiviral vector or with empty vector. a XR_111050 expression increased during osteogenic differentiation as evaluated by qRT-PCR. b The enhanced XR_111050 expression in BMSCs was verified with qRT-PCR. The increased XR_111050 expression enhanced mineralization of BMSCs as shown by c Alizarin red staining and d calcium quantitative analysis. qRT-PCR results showed that enhanced XR_111050 expression resulted in upregulated expressions of COL1A2 (e), OCN (f), BSP (g) and OPN (h) after osteogenic induction. i The enhanced XR_111050 expression increased RUNX2 expression in BMSCs. GAPDH was used as an internal control. Student’s t test was performed to determine statistical significance. Error bars represent SD (n = 3). *p < 0.05, **p < 0.01. BSP bone sialoprotein, COL1A2 collagen a-2(I) chain, d days, GADPH glyceraldehyde-3-phosphate dehydrogenase, OPN osteopontin, RUNX2 runt-related transcription factor 2, W weeks