Fig. 3

Density of terminal deoxynucleotidyl transferase 2′-deoxyuridine, 5′-triphosphate nick end labelling (TUNEL) apoptotic cells in the brain. Density of TUNEL cells was increased in the striatum (a), white matter tracts (b) and cerebral cortex (c) of LPS + hyperoxia animals. Representative figures showing no TUNEL-positive cells in a control animal (d) and increased TUNEL-positive cells (as indicated by black arrows) exhibiting nuclear condensation in an LPS + hyperoxia animal (e), which was reduced by hAECs (f). *p < 0.05; **p < 0.01; ***p < 0.001; ††LPS + hyperoxia versus control, p < 0.01; †††LPS + hyperoxia versus control, p < 0.001. Scale bar (d–f) = 30 μm. hAEC human amnion epithelial cell, LPS lipopolysaccharide